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Image Search Results
Journal: Molecular Medicine Reports
Article Title: Upregulation of lncRNA RMRP promotes the activation of cardiac fibroblasts by regulating miR-613
doi: 10.3892/mmr.2019.10634
Figure Lengend Snippet: RMRP interacts with miR-613 in cardiac fibroblasts. (A) An miRcode algorithm was used to predict RMRP-miRNA interaction. RMRP contains one seed sequence that can combine 6 miRNAs. (B) Effect of RMRP siRNA on the miR-613 and miR-1 expression in Ang-II-treated cardiac fibroblasts was assessed using reverse transcription-quantitative PCR. (C) Sequence alignment showing the complementarity between RMRP and miR-613. ‘|’ indicates base pairing. (D) Luciferase activity in cardiac fibroblasts co-transfected miR-613 mimic and luciferase reporters containing RMRP. (E) A diagram and the (F) results of MS2 RIP, performed to detect miR-613 endogenously associated with RMRP. Data are presented as the mean ± standard error of the mean. n=3 independent experiments. **P<0.01 vs. Con; ## P<0.01 vs. Ang-II; && P<0.01 vs. pmirGLO; $$ P<0.01 vs. MS2. miRNA, microRNA; siRNA, small interfering RNA; GFP, green fluorescent protein; Con, control; RMRP, mitochondrial RNA processing endoribonuclease; WT, wild-type; MUT, mutant; RIP, radioimmunoprecipitation; Ang II, angiotensin II.
Article Snippet: The fragment of RMRP containing the target sequence of miR-613 was inserted into a
Techniques: Sequencing, Expressing, Real-time Polymerase Chain Reaction, Luciferase, Activity Assay, Transfection, Small Interfering RNA, Mutagenesis